Michael W Kilgore, Ph.D.

Bio / Education: 

1980 B.S. Biology, University of Texas-Arlington
1984 M.S. Parasitology, University of Texas-Arlington
1990, Ph.D. Biochemistry/Molecular Biology, Texas Tech University Health Sciences Center
1994, Post-doc, Reproductive Endocrinology, UT Southwestern Medical Center
1994-2000, Associate Professor, Clemson University Department of Molecular Medicine

HONORS:
Teacher who made a difference award, University of Kentucky College of Education, 2014
Kentucky Equine Drug Research Council, Gubernatorial appointment, 2012.
Distinguished Alumni, Texas Tech University Health Sciences Center, 2005
Endocrine Society’s Select Abstract Award, Annual meeting, 2005.
Hot topics award, Jensen Symposia, December 2003.
Invited presentation at the Annual Director's meeting of the BIRCWH program, NIH, July 2003.
Invited speaker, Gordon Conference on Hormonal Carcinogenesis, 2001
Bridging Interdisciplinary Research Collaborations in Women’s Health Fellowship, 2001-2002
Sigma Xi Young Research Scientist of the Year, Clemson University, 2000
Endocrine Society’s Select Abstract Award, Annual meeting, 1999.
Presidential Plenary Session Award, The Society for Gynecological Investigation, 1997.
Presidential Scholars' Distinguished Teacher (Awarded by the US Secretary of Education) 1997.
Charles A. Hunter Prize Thesis Award, American Gynecological and Obstetrical Society, 1996.
National Institutes of Health, Post-doctoral Fellowship (1990-1993).
National Institutes of Health, Pre-doctoral Fellowship (1987-1989).

 

Research Description: 

Our lab has made key discoveries involving the role of PPAR1 in cancer including its overexpression in breast, ovarian, lung and prostate cancer. We were the first labs to clone human PPAR and have since defined the genomic structure of the gene. This led to changes in the proposed nomenclature and the identification of the distinct promoter elements that we have shown to drive tumor-specific expression. Our statistical approaches were successfully used to merge microarray analysis with computer based transcription factor binding databases to identify key factors mediating the promoter switching that drives the high levels of PPAR1 in breast cancer. I have collaborated on studies helping to define the role of PPAR1 in cardiovascular disease and neuronal function as well. I have extensive experience examining TZDs and individual fatty acids as activators of PPAR1 in breast and ovarian cancer. I have also employed molecular and pharmacological approaches to assess the posttranslational changes to PPAR1 and the role these play in ovarian and breast cancer cell growth, invasion and metastasis.
This expertise has resulted in collaboration with the Curry where we have focused on understanding PPAR’s role in ovarian function and demonstrated that TZDs have distinct actions on ovarian cancer cells. This lead to the discovery that TZD’s stimulate a distinct set of proteins in cancer cells, forming the foundation for the proposed studies. For this project I will use my extensive background in the transactivation of PPAR1 to assist with the exploration of TZD actions in the tumors and assist with the biomarker identification. I bring my molecular and pharmacologic background to dissect the mechanisms of TZD action on ovarian tumors to explore this exciting new paradigm that could offer new hope for the detection of early stage ovarian cancer.

PubMed Publications: 

  • Al-Alem, L.F.;McCord, L.A.;Southard, R.C.;Kilgore, M.W.;Curry TE, J. "Activation of the PKC pathway stimulates ovarian cancer cell proliferation, migration, and expression of MMP7 and MMP10." Biology of reproduction 89, 3 (2013): 73. [PubMed Link] | [ Full text ]
  • Zaytseva, Y.Y.;Wallis, N.K.;Southard, R.C.;Kilgore, M.W. "The PPARgamma antagonist T0070907 suppresses breast cancer cell proliferation and motility via both PPARgamma-dependent and -independent mechanisms." Anticancer research 31, 3 (2011): 813-23. [PubMed Link] | [ Full text ]
  • Al-Alem, L.;Southard, R.C.;Kilgore, M.W.;Curry, T.E. "Specific thiazolidinediones inhibit ovarian cancer cell line proliferation and cause cell cycle arrest in a PPARγ independent manner." PloS one 6, 1 (2011): e16179. [PubMed Link] | [ Full text ]
  • Pancani, T.;Phelps, J.T.;Searcy, J.L.;Kilgore, M.W.;Chen, K.C.;Porter, N.M.;Thibault, O. "Distinct modulation of voltage-gated and ligand-gated Ca2+ currents by PPAR-gamma agonists in cultured hippocampal neurons." Journal of neurochemistry 109, 6 (2009): 1800-11. [PubMed Link] | [ Full text ]
  • Wang, X.;Southard, R.C.;Allred, C.D.;Talbert, D.R.;Wilson, M.E.;Kilgore, M.W. "MAZ drives tumor-specific expression of PPAR gamma 1 in breast cancer cells." Breast cancer research and treatment 111, 1 (2008): 103-11. [PubMed Link] | [ Full text ]
  • Talbert, D.R.;Allred, C.D.;Zaytseva, Y.Y.;Kilgore, M.W. "Transactivation of ERalpha by Rosiglitazone induces proliferation in breast cancer cells." Breast cancer research and treatment 108, 1 (2008): 23-33. [PubMed Link] | [ Full text ]
  • Allred, C.D.;Talbert, D.R.;Southard, R.C.;Wang, X.;Kilgore, M.W. "PPARgamma1 as a molecular target of eicosapentaenoic acid in human colon cancer (HT-29) cells." The Journal of nutrition 138, 2 (2008): 250-6. [PubMed Link] | [ Full text ]
  • Zaytseva, Y.Y.;Wang, X.;Southard, R.C.;Wallis, N.K.;Kilgore, M.W. "Down-regulation of PPARgamma1 suppresses cell growth and induces apoptosis in MCF-7 breast cancer cells." Molecular cancer 7, (2008): 90. [PubMed Link] | [ Full text ]
  • Arzuaga, X.;Reiterer, G.;Majkova, Z.;Kilgore, M.W.;Toborek, M.;Hennig, B. "PPARalpha ligands reduce PCB-induced endothelial activation: possible interactions in inflammation and atherosclerosis." Cardiovascular toxicology 7, 4 (2007): 264-72. [PubMed Link] | [ Full text ]
  • Allred, C.D.;Kilgore, M.W. "Selective activation of PPARgamma in breast, colon, and lung cancer cell lines." Molecular and cellular endocrinology 235, 1-2 (2005): 21-9. [PubMed Link] | [ Full text ]
  • Wang, X.;Southard, R.C.;Kilgore, M.W. "The increased expression of peroxisome proliferator-activated receptor-gamma1 in human breast cancer is mediated by selective promoter usage." Cancer research 64, 16 (2004): 5592-6. [PubMed Link] | [ Full text ]
  • Jones, G.B.;Wright, J.M.;Hynd, G.;Wyatt, J.K.;Warner, P.M.;Huber, R.S.;Li, A.;Kilgore, M.W.;Sticca, R.P.;Pollenz, R.S. "Oxa-enediynes: probing the electronic and stereoelectronic contributions to the Bergman cycloaromatization." The Journal of organic chemistry 67, 16 (2002): 5727-32. [PubMed Link] | [ Full text ]
  • Wang, X.;Kilgore, M.W. "Signal cross-talk between estrogen receptor alpha and beta and the peroxisome proliferator-activated receptor gamma1 in MDA-MB-231 and MCF-7 breast cancer cells." Molecular and cellular endocrinology 194, 1-2 (2002): 123-33. [PubMed Link] | [ Full text ]
  • Jones, G.B.;Hynd, G.;Wright, J.M.;Purohit, A.;Plourde GW, 2nd;Huber, R.S.;Mathews, J.E.;Li, A.;Kilgore, M.W.;Bubley, G.J.;Yancisin, M.;Brown, M.A. "Target-directed enediynes: designed estramycins." The Journal of organic chemistry 66, 11 (2001): 3688-95. [PubMed Link] | [ Full text ]
  • Thoennes, S.R.;Tate, P.L.;Price, T.M.;Kilgore, M.W. "Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells." Molecular and cellular endocrinology 160, 1-2 (2000): 67-73. [PubMed Link] | [ Full text ]
  • Kilgore, M.W.;Tate, P.L.;Rai, S.;Sengoku, E.;Price, T.M. "MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response." Molecular and cellular endocrinology 129, 2 (1997): 229-35. [PubMed Link] | [ Full text ]
  • Demeter Arlotto, M.;Michael, M.D.;Kilgore, M.W.;Simpson, E.R. "17alpha-Hydroxylase gene expression in the bovine ovary: mechanisms regulating expression differ from those in adrenal cells." The Journal of steroid biochemistry and molecular biology 59, 1 (1996): 21-9. [PubMed Link] | [ Full text ]
  • O'Brien, S.N.;Mantzke, K.A.;Kilgore, M.W.;Price, T.M. "Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue." Analytical and quantitative cytology and histology 18, 2 (1996): 137-43. [PubMed Link] |
  • Michael, M.D.;Kilgore, M.W.;Morohashi, K.;Simpson, E.R. "Ad4BP/SF-1 regulates cyclic AMP-induced transcription from the proximal promoter (PII) of the human aromatase P450 (CYP19) gene in the ovary." The Journal of biological chemistry 270, 22 (1995): 13561-6. [PubMed Link] | [ Full text ]
  • Simpson, E.R.;Mahendroo, M.S.;Means, G.D.;Kilgore, M.W.;Hinshelwood, M.M.;Graham-Lorence, S.;Amarneh, B.;Ito, Y.;Fisher, C.R.;Michael, M.D. "Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis." Endocrine reviews 15, 3 (1994): 342-55. [PubMed Link] | [ Full text ]
  • Toda, K.;Simpson, E.R.;Mendelson, C.R.;Shizuta, Y.;Kilgore, M.W. "Expression of the gene encoding aromatase cytochrome P450 (CYP19) in fetal tissues." Molecular endocrinology (Baltimore, Md.) 8, 2 (1994): 210-7. [PubMed Link] | [ Full text ]
  • Bulun, S.E.;Rosenthal, I.M.;Brodie, A.M.;Inkster, S.E.;Zeller, W.P.;DiGeorge, A.M.;Frasier, S.D.;Kilgore, M.W.;Simpson, E.R. "Use of tissue-specific promoters in the regulation of aromatase cytochrome P450 gene expression in human testicular and ovarian sex cord tumors, as well as in normal fetal and adult gonads." The Journal of clinical endocrinology and metabolism 78, 2 (1994): 1616-21. [PubMed Link] | [ Full text ]
  • Simpson, E.R.;Mahendroo, M.S.;Means, G.D.;Kilgore, M.W.;Corbin, C.J.;Mendelson, C.R. "Tissue-specific promoters regulate aromatase cytochrome P450 expression." The Journal of steroid biochemistry and molecular biology 44, 4-6 (1993): 321-30. [PubMed Link] |
  • Simpson, E.R.;Mahendroo, M.S.;Means, G.D.;Kilgore, M.W.;Corbin, C.J.;Mendelson, C.R. "Tissue-specific promoters regulate aromatase cytochrome P450 expression." Clinical chemistry 39, 2 (1993): 317-24. [PubMed Link] | [ Full text ]
  • Bulun, S.E.;Rosenthal, I.M.;Brodie, A.M.;Inkster, S.E.;Zeller, W.P.;DiGeorge, A.M.;Frasier, S.D.;Kilgore, M.W.;Simpson, E.R. "Use of tissue-specific promoters in the regulation of aromatase cytochrome P450 gene expression in human testicular and ovarian sex cord tumors, as well as in normal fetal and adult gonads." The Journal of clinical endocrinology and metabolism 77, 6 (1993): 1616-21. [PubMed Link] | [ Full text ]
  • Kilgore, M.W.;Means, G.D.;Mendelson, C.R.;Simpson, E.R. "Alternative promotion of aromatase P-450 expression in the human placenta." Molecular and cellular endocrinology 83, 1 (1992): R9-16. [PubMed Link] | [ Full text ]
  • Simpson, E.R.;Kilgore, M.W.;Mahendroo, M.S.;Means, G.D.;Corbin, C.J.;Mendelson, C.R. "Regulation of human aromatase cytochrome P450 gene expression." The Journal of steroid biochemistry and molecular biology 43, 8 (1992): 923-30. [PubMed Link] | [ Full text ]
  • Lephart, E.D.;Simpson, E.R.;McPhaul, M.J.;Kilgore, M.W.;Wilson, J.D.;Ojeda, S.R. "Brain aromatase cytochrome P-450 messenger RNA levels and enzyme activity during prenatal and perinatal development in the rat." Brain research. Molecular brain research 16, 3-4 (1992): 187-92. [PubMed Link] |
  • Means, G.D.;Kilgore, M.W.;Mahendroo, M.S.;Mendelson, C.R.;Simpson, E.R. "Tissue-specific promoters regulate aromatase cytochrome P450 gene expression in human ovary and fetal tissues." Molecular endocrinology (Baltimore, Md.) 5, 12 (1991): 2005-13. [PubMed Link] | [ Full text ]
  • Kilgore, M.W.;Rommerts, F.F.;Wirtz, K.W.;Stocco, D.M. "Regulation of steroidogenesis in subclones of the MA-10 mouse Leydig tumor cell line." Molecular and cellular endocrinology 69, 1 (1990): 9-16. [PubMed Link] | [ Full text ]
  • Doris, P.A.;Kilgore, M.W.;Durham, D.;Alberts, D.;Stocco, D.M. "An endogenous digitalis-factor derived from the adrenal gland: studies of adrenocortical tumor cells." Endocrinology 125, 5 (1989): 2580-6. [PubMed Link] | [ Full text ]
  • Kilgore, M.W.;Stocco, D.M. "Initial characterization of a subclone of the MA-10 mouse Leydig tumor cell line." Endocrinology 124, 3 (1989): 1210-6. [PubMed Link] | [ Full text ]
  • Stocco, D.M.;Kilgore, M.W. "Induction of mitochondrial proteins in MA-10 Leydig tumour cells with human choriogonadotropin." The Biochemical journal 249, 1 (1988): 95-103. [PubMed Link] | [ Full text ]
  • Kilgore, M.W.;Stewart, G.L.;Smatresk, N.J. "Oxygen uptake in mice infected with Trichinella spiralis." The Journal of parasitology 74, 4 (1988): 721-4. [PubMed Link] |
  • Kilgore, M.W.;Stewart, G.L.;Lou, M. "Chemical composition of newborn larvae, muscle larvae and adult Trichinella spiralis." International journal for parasitology 16, 5 (1986): 455-60. [PubMed Link] | [ Full text ]
  • Stewart, G.L.;Raines, K.M.;Kilgore, M.W. "Glucose absorption in vitro by the enteric stages of Trichinella spiralis." Parasitology 93 ( Pt 3), (1986): 581-6. [PubMed Link] |
  • Green, J.;Driber, T.J.;Deshmukh, V.;Nealis, J.G.;Noran, W.;Miller, E.;Kilgore MW, 2nd "Brain stem auditory evoked responses in clinical practice." Clinical EEG (electroencephalography) 11, 1 (1980): 28-33. [PubMed Link] |
  • Busby, S.;Green, J.;Haycook, W.;Kilgore MW, 2nd "Hemiatrophy of the tongue: a rare complication of the hemiplegic migraine." The Journal of clinical psychiatry 39, 2 (1978): 166-7. [PubMed Link] |