Ming Cui Gong, Ph.D., M.D.

Bio / Education: 

Ph.D. Peking Union Medical College, China and University of Virginia 1994

Research Description: 

Current research in our laboratory focuses on vascular mechanisms of type 2 diabetes associated hypertension and blood pressure circadian rhythm disruptions

More than 170 million people worldwide have diabetes. Hypertension occurs more frequently in these diabetic patients than in those without diabetes. Moreover, when hypertension is superimposed on diabetes, the progression of diabetic complications becomes significantly more severe. However, the mechanisms by which diabetes cause an increase in the incidence of hypertension are not well understood. We and others have found that type 2 diabetic mouse models, db/db mice and high fat-diet fed mice, are hypertensive and vascular smooth muscle tissues isolated from them manifest contractile hyper-reactivity. One of our goals is to elucidate the molecular mechanisms underlying such vascular smooth muscle hyper-reactivity and its role in diabetes associated hypertension. Currently, we are focusing on the role of CPI-17, a myosin phosphatase inhibitory protein that is preferentially expressed in smooth muscle and plays an important role in regulating physiological smooth muscle contraction. We found that CPI-17 is activated and up-regulated in vascular smooth muscle of type 2 diabetic mice.

Blood pressure has a circadian pattern characterized by a low period during sleep; an early morning, post awakening rise; and a high plateau period while the subject is awake. There is increasing evidence that not only does the average level of blood pressure, but particularly an abnormal circadian blood pressure rhythm with a decreased fall in night time blood pressure, determine the development of diabetic complications and cardiovascular outcomes. The mechanisms that account for the abnormal blood pressure diurnal pattern in type 2 diabetes remain largely unknown. We are currently exploring the role of dysregulated core clock genes and vascular smooth muscle contractile diurnal variations in diabetes associated blood pressure circadian rhythm disruptions.

Combinations of the state-of-the-art techniques with classical physiological and biochemical methods were used to pursue our goals. These include radiotelemetry monitoring mouse blood pressure, smooth muscle specific transgenic and knockout mouse models, isometric contraction determination with isolated vascular tissues, microarray, real-time PCR, small interference RNA and adenoviral mediated gene transfer.

Grants: 

RO1 HL 106843 and RO1 HL125228

Selected Publications: 

Wen Su, Zhenheng Guo, David C. Randall, Lisa A. Cassis, David R. Brown, Ming C. Gong (2008) Hypertension and Disrupted Blood Pressure Circadian Rhythm in Type 2 Diabetic db/db Mice. American Journal of Physiology-Heart and Circulation, 295: H1634-H1641

Zhongwen Xie, Ming C. Gong, Wen Su, Dongping Xie, John Turk, and Zhenheng Guo (2010) Role of calcium-independent phospholipase A2ß in high glucose-induced activation of RhoA, Rho-kinase, and CPI-17 in cultured vascular smooth msucle cells and vascular smooth muscle hypercontractility in diabeteic animals. Journal of Biological Chemistry, 285 (12): 8628-38

Wen Su, Zhongwen Xie, Zhenheng Guo, Marilyn J. Duncan, Jenny Lutshumba, and Ming C. Gong (2012) Altered clock gene expression and vascular smooth muscle diurnal contractile variations in type 2 diabetic db/db mice. American Journal of Physiology-Heart and Circulation, 302(3): H621-33

Lindsay E. Calderon, Shu Liu, Wen Su, Zhenheng Guo, Wanda Eberhard, and Ming C. Gong (2012) iPLA2ß overexpression in smooth muscle exacerbates angiotensin II-induced blood pressure increase and vascular remodeling. PLos One 7(2): e31850

Shu Liu, Zhongwen Xie, Qingwei Zhao, Huan Pang, Lindsay E. Calderon, Ming C. Gong, and Zhenheng Guo (2012) Smooth muscle specific expression of calcium independent phospholipase A2ß exacerbates neointinal formation in mice. The Journal of Biological Chemistry 287(29): 24739-53

Wen Su, Zhongwen Xie, Shu Liu, Lindsay Calderon, Zhenheng Guo, and Ming C. Gong (2013) Smooth muscle specific CPI-17 over-expression increases contractile responses and blood pressure. The American Journal of Physiology-Heart and Circulation 304 (1): H104-13

Shu Liu, Zhongwen Xie, Alan Daugherty, Lisa Cassis, Kevin Pearson, Ming C. Gong, and Zhenheng Guo (2013) Mineralocorticoid receptor agonists induce mouse aortic aneurysm formation and rupture in the presence of high salt. Arterioscler. Thromb. Vasc. Biol. 33 (7): 1568-79

Zhongwen Xie, Wen Su, Shu Liu, Guogang Zhao, Karyn Esser, Elizabeth A. Schroder, Mellani Lefta, Harald M. Stauss, Zhenheng Guo, and Ming Cui Gong (2015) Smooth muscle BMAL1 participates in blood pressure circadian rhythm regulation. Journal of Clinical Investigation  125(1): 324-336

Other Publications

PubMed Publications: 

  • Haggerty, C.M.;Mattingly, A.C.;Gong, M.C.;Su, W.;Daugherty, A.;Fornwalt, B.K. "Telemetric Blood Pressure Assessment in Angiotensin II-Infused ApoE-/- Mice: 28 Day Natural History and Comparison to Tail-Cuff Measurements." PloS one 10, 6 (2015): e0130723. [PubMed Link] | [ Full text ]
  • Xie, Z.;Su, W.;Liu, S.;Zhao, G.;Esser, K.;Schroder, E.A.;Lefta, M.;Stauss, H.M.;Guo, Z.;Gong, M.C. "Smooth-muscle BMAL1 participates in blood pressure circadian rhythm regulation." The Journal of clinical investigation 125, 1 (2015): 324-36. [PubMed Link] | [ Full text ]
  • Wang, S.N.;Lan, L.;Hua, W.B.;Shi, Z.H.;Chen, Y.Q.;Gong, M.C.;Zhong, L. "Ce-Zr-La/Al2O3 prepared in a continuous stirred-tank reactor: a highly thermostable support for an efficient Rh-based three-way catalyst." Dalton transactions (Cambridge, England : 2003) 44, 47 (2015): 20484-92. [PubMed Link] | [ Full text ]
  • Su, W.;Xie, Z.;Liu, S.;Calderon, L.E.;Guo, Z.;Gong, M.C. "Smooth muscle-selective CPI-17 expression increases vascular smooth muscle contraction and blood pressure." American journal of physiology. Heart and circulatory physiology 305, 1 (2013): H104-13. [PubMed Link] | [ Full text ]
  • Liu, S.;Xie, Z.;Daugherty, A.;Cassis, L.A.;Pearson, K.J.;Gong, M.C.;Guo, Z. "Mineralocorticoid receptor agonists induce mouse aortic aneurysm formation and rupture in the presence of high salt." Arteriosclerosis, thrombosis, and vascular biology 33, 7 (2013): 1568-79. [PubMed Link] | [ Full text ]
  • Anigbogu, C.N.;Speakman, R.O.;Silcox, D.L.;Brown, L.V.;Brown, D.R.;Gong, M.C.;Patwardhan, A.R.;Reynolds, L.R.;Karounos, D.G.;Burgess, D.E.;Baldridge, B.R.;Randall, D.C. "Extended longitudinal analysis of arterial pressure and heart rate control in unanesthetized rats with type 1 diabetes." Autonomic neuroscience : basic & clinical 170, 1-2 (2012): 20-9. [PubMed Link] | [ Full text ]
  • Liu, S.;Xie, Z.;Zhao, Q.;Pang, H.;Turk, J.;Calderon, L.;Su, W.;Zhao, G.;Xu, H.;Gong, M.C.;Guo, Z. "Smooth muscle-specific expression of calcium-independent phospholipase A2β (iPLA2β) participates in the initiation and early progression of vascular inflammation and neointima formation." The Journal of biological chemistry 287, 29 (2012): 24739-53. [PubMed Link] | [ Full text ]
  • Su, W.;Xie, Z.;Guo, Z.;Duncan, M.J.;Lutshumba, J.;Gong, M.C. "Altered clock gene expression and vascular smooth muscle diurnal contractile variations in type 2 diabetic db/db mice." American journal of physiology. Heart and circulatory physiology 302, 3 (2012): H621-33. [PubMed Link] | [ Full text ]
  • Calderon, L.E.;Liu, S.;Su, W.;Xie, Z.;Guo, Z.;Eberhard, W.;Gong, M.C. "iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling." PloS one 7, 2 (2012): e31850. [PubMed Link] | [ Full text ]
  • Garcia, J.A.;Stephenson, A.J.;Ireland, J.;Finke, J.;Elson, P.;Schaaf, L.;Gong, M.C.;Campbell, S.;Dreicer, R. "Sunitinib in BCG-refractory non-muscle-invasive transitional cell carcinoma of the bladder." Journal of clinical oncology : official journal of the American Society of Clinical Oncology 29, 7_suppl (2011): 262. [PubMed Link] |
  • Randall, D.C.;Speakman, R.O.;Silcox, D.L.;Brown, L.V.;Brown, D.R.;Gong, M.C.;Patwardhan, A.;Reynolds, L.R.;Karounos, D.G.;Burgess, D.E.;Anigbogu, C.N. "Longitudinal analysis of arterial blood pressure and heart rate response to acute behavioral stress in rats with type 1 diabetes mellitus and in age-matched controls." Frontiers in physiology 2, (2011): 53. [PubMed Link] | [ Full text ]
  • Xie, Z.;Gong, M.C.;Su, W.;Xie, D.;Turk, J.;Guo, Z. "Role of calcium-independent phospholipase A2beta in high glucose-induced activation of RhoA, Rho kinase, and CPI-17 in cultured vascular smooth muscle cells and vascular smooth muscle hypercontractility in diabetic animals." The Journal of biological chemistry 285, 12 (2010): 8628-38. [PubMed Link] | [ Full text ]
  • Bridges, P.J.;Jo, M.;Al Alem, L.;Na, G.;Su, W.;Gong, M.C.;Jeoung, M.;Ko, C. "Production and binding of endothelin-2 (EDN2) in the rat ovary: endothelin receptor subtype A (EDNRA)-mediated contraction." Reproduction, fertility, and development 22, 5 (2010): 780-7. [PubMed Link] | [ Full text ]
  • Chapman, D.M.;Pohar, K.S.;Gong, M.C.;Bahnson, R.R. "Preoperative hydronephrosis as an indicator of survival after radical cystectomy." Urologic oncology 27, 5 (2009): 491-5. [PubMed Link] | [ Full text ]
  • Gupte, M.;Boustany-Kari, C.M.;Bharadwaj, K.;Police, S.;Thatcher, S.;Gong, M.C.;English, V.L.;Cassis, L.A. "ACE2 is expressed in mouse adipocytes and regulated by a high-fat diet." American journal of physiology. Regulatory, integrative and comparative physiology 295, 3 (2008): R781-8. [PubMed Link] | [ Full text ]
  • Su, W.;Guo, Z.;Randall, D.C.;Cassis, L.;Brown, D.R.;Gong, M.C. "Hypertension and disrupted blood pressure circadian rhythm in type 2 diabetic db/db mice." American journal of physiology. Heart and circulatory physiology 295, 4 (2008): H1634-41. [PubMed Link] | [ Full text ]
  • Richardson, D.L.;Seamon, L.G.;Gong, M.C.;Chapman, D.M.;Cohn, D.E. "Panniculectomy concurrent with anterior pelvic exenteration for recurrent cervical cancer." Gynecologic oncology 108, 2 (2008): 449-51. [PubMed Link] | [ Full text ]
  • Nikolic, D.M.;Gong, M.C.;Turk, J.;Post, S.R. "Class A scavenger receptor-mediated macrophage adhesion requires coupling of calcium-independent phospholipase A(2) and 12/15-lipoxygenase to Rac and Cdc42 activation." The Journal of biological chemistry 282, 46 (2007): 33405-11. [PubMed Link] | [ Full text ]
  • Al-Alem, L.;Bridges, P.J.;Su, W.;Gong, M.C.;Iglarz, M.;Ko, C. "Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats." The Journal of endocrinology 193, 3 (2007): 383-91. [PubMed Link] | [ Full text ]
  • Xie, Z.;Gong, M.C.;Su, W.;Turk, J.;Guo, Z. "Group VIA phospholipase A2 (iPLA2beta) participates in angiotensin II-induced transcriptional up-regulation of regulator of g-protein signaling-2 in vascular smooth muscle cells." The Journal of biological chemistry 282, 35 (2007): 25278-89. [PubMed Link] | [ Full text ]
  • Esser, K.A.;Su, W.;Matveev, S.;Wong, V.;Zeng, L.;McCarthy, J.J.;Smart, E.J.;Guo, Z.;Gong, M.C. "Voluntary wheel running ameliorates vascular smooth muscle hyper-contractility in type 2 diabetic db/db mice." Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 32, 4 (2007): 711-20. [PubMed Link] | [ Full text ]
  • Nikolic, D.M.;Cholewa, J.;Gass, C.;Gong, M.C.;Post, S.R. "Class A scavenger receptor-mediated cell adhesion requires the sequential activation of Lyn and PI3-kinase." American journal of physiology. Cell physiology 292, 4 (2007): C1450-8. [PubMed Link] | [ Full text ]
  • Wang, J.L.;Zhong, J.B.;Gong, M.C.;Liu, Z.M.;Ming, Z.;Chen, Y.Q. "Remove cooking fume using catalytic combustion over Pt/La-Al2O3." Journal of environmental sciences (China) 19, 6 (2007): 644-6. [PubMed Link] |
  • Pang, H.;Guo, Z.;Xie, Z.;Su, W.;Gong, M.C. "Divergent kinase signaling mediates agonist-induced phosphorylation of phosphatase inhibitory proteins PHI-1 and CPI-17 in vascular smooth muscle cells." American journal of physiology. Cell physiology 290, 3 (2006): C892-9. [PubMed Link] | [ Full text ]
  • Xie, Z.;Su, W.;Guo, Z.;Pang, H.;Post, S.R.;Gong, M.C. "Up-regulation of CPI-17 phosphorylation in diabetic vasculature and high glucose cultured vascular smooth muscle cells." Cardiovascular research 69, 2 (2006): 491-501. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Arbogast, S.;Guo, Z.;Mathenia, J.;Su, W.;Reid, M.B. "Calcium-independent phospholipase A2 modulates cytosolic oxidant activity and contractile function in murine skeletal muscle cells." Journal of applied physiology (Bethesda, Md. : 1985) 100, 2 (2006): 399-405. [PubMed Link] | [ Full text ]
  • Ko, C.;Gieske, M.C.;Al-Alem, L.;Ahn, Y.H.;Su, W.;Gong, M.C.;Iglarz, M.;Koo, Y. "Endothelin-2 in ovarian follicle rupture." Endocrinology 147, 4 (2006): 1770-9. [PubMed Link] | [ Full text ]
  • Guo, Z.;Su, W.;Allen, S.;Pang, H.;Daugherty, A.;Smart, E.;Gong, M.C. "COX-2 up-regulation and vascular smooth muscle contractile hyperreactivity in spontaneous diabetic db/db mice." Cardiovascular research 67, 4 (2005): 723-35. [PubMed Link] | [ Full text ]
  • Pang, H.;Guo, Z.;Su, W.;Xie, Z.;Eto, M.;Gong, M.C. "RhoA-Rho kinase pathway mediates thrombin- and U-46619-induced phosphorylation of a myosin phosphatase inhibitor, CPI-17, in vascular smooth muscle cells." American journal of physiology. Cell physiology 289, 2 (2005): C352-60. [PubMed Link] | [ Full text ]
  • Cassis, L.A.;Huang, J.;Gong, M.C.;Daugherty, A. "Role of metabolism and receptor responsiveness in the attenuated responses to Angiotensin II in mice compared to rats." Regulatory peptides 117, 2 (2004): 107-16. [PubMed Link] | [ Full text ]
  • Brentjens, R.J.;Latouche, J.B.;Santos, E.;Marti, F.;Gong, M.C.;Lyddane, C.;King, P.D.;Larson, S.;Weiss, M.;Rivière, I.;Sadelain, M. "Eradication of systemic B-cell tumors by genetically targeted human T lymphocytes co-stimulated by CD80 and interleukin-15." Nature medicine 9, 3 (2003): 279-86. [PubMed Link] | [ Full text ]
  • Su, W.;Guo, Z.;Deschepper, C.F.;Randall, D.C.;Gong, M.C. "Dissociation of coronary artery contractile hyperreactivity from hypertension." American journal of hypertension 16, 7 (2003): 570-6. [PubMed Link] |
  • Guo, Z.;Su, W.;Ma, Z.;Smith, G.M.;Gong, M.C. "Ca2+-independent phospholipase A2 is required for agonist-induced Ca2+ sensitization of contraction in vascular smooth muscle." The Journal of biological chemistry 278, 3 (2003): 1856-63. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Gorenne, I.;Read, P.;Jia, T.;Nakamoto, R.K.;Somlyo, A.V.;Somlyo, A.P. "Regulation by GDI of RhoA/Rho-kinase-induced Ca2+ sensitization of smooth muscle myosin II." American journal of physiology. Cell physiology 281, 1 (2001): C257-69. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Fair, W.R. "Introduction--translational research trends: urologic gene therapy." Molecular urology 4, 2 (2000): 37-8. [PubMed Link] |
  • Fu, X.;Gong, M.C.;Jia, T.;Somlyo, A.V.;Somlyo, A.P. "The effects of the Rho-kinase inhibitor Y-27632 on arachidonic acid-, GTPgammaS-, and phorbol ester-induced Ca2+-sensitization of smooth muscle." FEBS letters 440, 1-2 (1998): 183-7. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Fujihara, H.;Walker, L.A.;Somlyo, A.V.;Somlyo, A.P. "Down-regulation of G-protein-mediated Ca2+ sensitization in smooth muscle." Molecular biology of the cell 8, 2 (1997): 279-86. [PubMed Link] | [ Full text ]
  • Fujihara, H.;Walker, L.A.;Gong, M.C.;Lemichez, E.;Boquet, P.;Somlyo, A.V.;Somlyo, A.P. "Inhibition of RhoA translocation and calcium sensitization by in vivo ADP-ribosylation with the chimeric toxin DC3B." Molecular biology of the cell 8, 12 (1997): 2437-47. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Fujihara, H.;Somlyo, A.V.;Somlyo, A.P. "Translocation of rhoA associated with Ca2+ sensitization of smooth muscle." The Journal of biological chemistry 272, 16 (1997): 10704-9. [PubMed Link] | [ Full text ]
  • Gailly, P.;Gong, M.C.;Somlyo, A.V.;Somlyo, A.P. "Possible role of atypical protein kinase C activated by arachidonic acid in Ca2+ sensitization of rabbit smooth muscle." The Journal of physiology 500 ( Pt 1), (1997): 95-109. [PubMed Link] | [ Full text ]
  • Jensen, P.E.;Gong, M.C.;Somlyo, A.V.;Somlyo, A.P. "Separate upstream and convergent downstream pathways of G-protein- and phorbol ester-mediated Ca2+ sensitization of myosin light chain phosphorylation in smooth muscle." The Biochemical journal 318 ( Pt 2), (1996): 469-75. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Iizuka, K.;Nixon, G.;Browne, J.P.;Hall, A.;Eccleston, J.F.;Sugai, M.;Kobayashi, S.;Somlyo, A.V.;Somlyo, A.P. "Role of guanine nucleotide-binding proteins--ras-family or trimeric proteins or both--in Ca2+ sensitization of smooth muscle." Proceedings of the National Academy of Sciences of the United States of America 93, 3 (1996): 1340-5. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Kinter, M.T.;Somlyo, A.V.;Somlyo, A.P. "Arachidonic acid and diacylglycerol release associated with inhibition of myosin light chain dephosphorylation in rabbit smooth muscle." The Journal of physiology 486 ( Pt 1), (1995): 113-22. [PubMed Link] | [ Full text ]
  • Geary, E.S.;Gong, M.C.;Shortliffe, L.M. "Biology and treatment of pediatric genitourinary tumors." Current opinion in oncology 6, 3 (1994): 292-300. [PubMed Link] |
  • Gong, M.C.;Fuglsang, A.;Alessi, D.;Kobayashi, S.;Cohen, P.;Somlyo, A.V.;Somlyo, A.P. "Arachidonic acid inhibits myosin light chain phosphatase and sensitizes smooth muscle to calcium." The Journal of biological chemistry 267, 30 (1992): 21492-8. [PubMed Link] | [ Full text ]
  • Gong, M.C.;Cohen, P.;Kitazawa, T.;Ikebe, M.;Masuo, M.;Somlyo, A.P.;Somlyo, A.V. "Myosin light chain phosphatase activities and the effects of phosphatase inhibitors in tonic and phasic smooth muscle." The Journal of biological chemistry 267, 21 (1992): 14662-8. [PubMed Link] | [ Full text ]
  • Kobayashi, S.;Gong, M.C.;Somlyo, A.V.;Somlyo, A.P. "Ca2+ channel blockers distinguish between G protein-coupled pharmacomechanical Ca2+ release and Ca2+ sensitization." The American journal of physiology 260, 2 Pt 1 (1991): C364-70. [PubMed Link] | [ Full text ]
  • Somlyo, A.P.;Kitazawa, T.;Kobayashi, S.;Gong, M.C.;Somlyo, A.V. "Pharmacomechanical coupling: the membranes talk to the crossbridges." Advances in experimental medicine and biology 304, (1991): 185-208. [PubMed Link] |
  • Xu, Z.B.;Wu, J.;Chen, H.C.;Lu, Y.J.;Gong, M.C. "Radix Codonopsis pilosulae and radix Astragali promote erythrocyte glycolysis." Chinese medical journal 100, 8 (1987): 654-7. [PubMed Link] |
  • Gong, M.C. " Preliminary observations of the effect of fominoben on the ventilation, arterial oxygen pressure and arterial pH in the rabbit under hypoxia (author's transl) ." Zhonghua jie he he hu xi xi ji bing za zhi = Chinese journal of tuberculosis and respiratory diseases 4, 3 (1981): 151-2. [PubMed Link] |